Protocols

The following protocols have been obtained and adapted from several sources:

 

• Bioanalyzer


• Bioanalyzer Operation


• CDM media


• Protocol for CDM


• Fragment Analysis


• Fragment Analysis Sample Setup


• Library Building


• Cracking Gel Buffer
• Cracking Gel Procedure
• Electroporation
• Nebulizing DNA


• Microarrays


• Affymetrix Array Protocol
• CombiMatrix Hybridization Protocol
• DNAse Treatment
• Macroarray Printing
• Macroarray Printing: Biomek template
• Mirus Label IT µArray Biotin Labeling Protocol


• Nanodrop


• Nanodrop Operation


• PCRs


• TTH PCR Protocol
• Taq PCR Protocol


• PF2D proteomics


• Beckman Proteomelab PF2D Kit Protocol
• Brain Tissue Protocol: Freon
• Brain Tissue Protocol: IPA Hex
• Compositions of the PF2D Buffers
• Preparing sample for Mass Spec at PRS
• Prepping Samples for PF2D
• Running the PF2D
• Submitting Samples to PRS
• Transfer of PF2D 2nd Fractions for MS Analysis


• Picking Colonies and Plasmid Prep


• G3 Colony Picker Protocol
• Millipore Plasmid Prep: Centrifuge Method
• 96 well Millipore Prep using Vacuum Manifold
• SeqPrep 384 plasmid prep


• Real Time


• cDNA Synthesis for Real Time
• Real Time Primer Efficiencies


• RNA Isolation


• Hot Phenol RNA Isolation


• Sequencing Reactions


• Ethanol_Precipitation_of_Sequencing_Reactions
• Genome Sequencing Projects/Facility Prepped Template
• Small Scale Customer Sequencing Genomic DNA Protocol
• Customer Sequencing Reaction Cleanup: SDS treatment
• Small Scale Customer Sequencing: STeP 10µl Reaction
• Small Scale Customer Sequencing: GC rich Templates/Betaine Protocol